Linear Polyethylenimine (PEI)
Linear PEI for transfection, viral vector production and recombinant protein expression. Available in R&D and GMP grades, with scalable manufacturing and powder or solution formats.
About Curapath® PEI
Curapath® PEI (linear polyethylenimine Hydrochloride, MW 20,000 Da) forms stable complexes with DNA, supporting efficient nucleic acid delivery in applications ranging from in vitro cell transfection to viral vector production and recombinant protein expression.
Available as a dry form or solution in R&D and GMP grades, Curapath PEI® supports the transition from process development to clinical and commercial manufacturing. Its optimized formulation enables high-titer vector and recombinant protein production while maintaining cell viability, offering a reliable and scalable alternative to conventional PEI-based transfection reagents.
Commonly Used For
Linear PEI is a versatile transfection reagent widely used in research, process development, and bioproduction workflows. It is particularly valuable for plasmid delivery in mammalian cells, supporting both exploratory R&D and scalable upstream applications.
Transient Expression and Stable Cell Line Development
PEI is commonly used for transient transfection in mammalian cells and for the generation of stable producer cell lines, enabling efficient gene delivery during early discovery and cell line development workflows.
Co-Transfection and Sequential Transfection Workflows
Its flexibility makes PEI suitable for co-transfection strategies and sequential transfection protocols, helping researchers introduce multiple plasmids or optimize multistep expression workflows in vitro.
AAV and Lentiviral Vector Production
PEI is widely adopted for plasmid delivery during AAV and lentiviral vector production, supporting efficient transfection of producer cells in workflows used for gene therapy and advanced therapy development.
Recombinant Protein and Monoclonal Antibody (mAb) Expression
PEI is frequently used in recombinant protein and monoclonal antibody expression systems, offering a practical and scalable option for upstream transfection in biologics development and production.
Curapath® PEI Product Overview
Request Curapath® PEI
Select the Curapath PEI® format that best fits your application and submit the corresponding product request. Our team will review your requirements and contact you directly.
Request Dry Curapath® PEI
Complete the form below to request Curapath PEI® in dry format. Our team will review your application, grade and supply requirements.
Request Curapath® PEI Solution
Complete the form below to request Curapath PEI® in solution format. Our team will review your application, grade and supply requirements.
Frequently asked questions about linear PEI
What is Curapath
Curapath is a European Contract Development and Manufacturing Organization (CDMO) specialized in non-viral drug delivery systems. We support biotech and pharmaceutical companies from early development through GMP-ready and commercial manufacturing, combining proprietary delivery components, in-house technologies, and deep expertise in complex formulation and CMC development.
What is linear polyethylenimine hydrochloride (linear PEI)?
Linear polyethylenimine hydrochloride, commonly called linear PEI, is a cationic polymer widely used as a transfection reagent. It forms complexes with nucleic acids such as plasmid DNA, helping deliver them into mammalian cells for gene expression, viral vector production and recombinant protein production.
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What is linear PEI used for?
Linear PEI is used for plasmid DNA delivery in mammalian-cell workflows, including transient transfection, stable cell line development, co-transfection and sequential transfection. It is also widely used in AAV and lentiviral vector production, as well as recombinant protein and monoclonal antibody expression.
Can linear PEI be used for AAV and lentiviral vector production?
Yes. Linear PEI is commonly used to transfect producer cells with the plasmids required for AAV and lentiviral vector production. Optimising the DNA-to-PEI ratio, cell density and transfection conditions is important to achieve the best balance between transfection efficiency, cell viability and vector yield for each process.
Is Curapath® PEI available in R&D and GMP grades?
Yes. Curapath® PEI is available in R&D and GMP grades to support the progression from early process development to clinical and commercial manufacturing. Using a comparable material across development stages can help reduce changes during scale-up and technology transfer.
Is Curapath® PEI available as a powder or a solution?
Curapath® PEI is available in both dry and solution formats. The most suitable format depends on your workflow, internal preparation requirements, storage preferences and intended stage of development.
How can I select the right PEI format and grade for my process?
The appropriate format and grade depend on the application, cell system, development stage, supply requirements and manufacturing strategy. Share your intended use, expected scale and preferred format with the Curapath team through the product request form so we can help identify the most suitable option.
